Electrophoretic protein patterns of three species ofPhytophthora associated with black pod disease of cocoa (Theobroma cacao L.) |
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Authors: | P Chowdappa R Chandramohanan |
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Institution: | (1) Central Plantation Crops Research Institute, Regional Station, 574 243 Vittal, India |
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Abstract: | When electrophoretic profiles of native proteins from vegetative mycelia ofPhytophthora palmivora, Phytophthora capsici and Phytophthora citrophthora causing black pod disease of cocoa in India were compared on a single Polyacrylamide gel, the isolates of same species were
readily distinguished both qualitatively by visual similarity in banding patterns and quantitatively by calculating similarity
coefficients. Similarity coefficients were generally much higher between isolates within a species than between isolates of
different species. The dendrograms obtained after unweighted pair grouping with arithmetic averaging cluster analysis, revealed
that all the isolates ofPhytophthora capsici were highly homogenous and formed a single cluster. The isolates ofPhytophthora citrophthora were resolved into two electrophoretic types which were clustered into two distinct sub groups.Phytophthora palmivora formed a separate group. Thus, the results reveal that polyacrylamide gel electrophoresis can be used successfully in distinguishing
species and sub groups within a species ofPhytophthora encountered on cocoa.
CPCRl contribution No. 914. |
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Keywords: | Phytophthora palmivora Phytophthora capsici Phytophthora citrophthora electrophoretic protein profiles |
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