DspA from Strongylocentrotus purpuratus: The first biochemically characterized haloalkane dehalogenase of non-microbial origin |
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| Authors: | Andrea Fortova Eva Sebestova Veronika Stepankova Tana KoudelakovaLenka Palkova Jiri Damborsky Radka Chaloupkova |
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| Institution: | Loschmidt Laboratories, Department of Experimental Biology and Research Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic |
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| Abstract: | Haloalkane dehalogenases are known as bacterial enzymes cleaving a carbon–halogen bond in halogenated compounds. Here we report the first biochemically characterized non-microbial haloalkane dehalogenase DspA from Strongylocentrotus purpuratus. The enzyme shows a preference for terminally brominated hydrocarbons and enantioselectivity towards β-brominated alkanes. Moreover, we identified other putative haloalkane dehalogenases of eukaryotic origin, representing targets for future experiments to discover dehalogenases with novel catalytic properties. |
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| Keywords: | Eukaryotic haloalkane dehalogenase Catalytic activity Substrate specificity Enantioselectivity Gene mining |
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