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| Characterization of 5‘—proximal sequence of mouse GABA transporter gene （GAT—1） | |
| 引用本文： | FEIJIAN FANGHUANG 等. Characterization of 5‘—proximal sequence of mouse GABA transporter gene （GAT—1）[J]. Cell research, 1997, 7(1): 61-67 |
| 作者姓名： | FEIJIAN FANGHUANG 等 |
| 作者单位： | [1]ShanghaiInstituteofCellBiology,ChineseAcademyofSciences,Shanghai200031 [2]ShanghaiInstituteofCellBiology,ChineseAcademyofSciences,Shanghai200031 |
| 摘 要： | The cDNA molecule encoding the mouse GABA transporter gene(GAT-1) was used as probe for selecting GAT-1 gene from mouse genomic library.A positive clone,harboring the whole open reading frame of the GAT-1 protein and designated as MGABAT-G,was fished out from the library,the 5‘ proximal region and intron 1 were sequenced and analysed,and low homology was found in the above region between GAT-1 genes from mouse and human except some short conserved sequences.The DNA-protein interactions between DNA fragments containing the conserved sequences in the 5‘ proximal region and nuclear proteins from different tissues of mouse were studied by means of gel-shift assay,and Southern-Western blot.The results indicate a possible positive-negative regulation mode controlling the expression of the mouse GAT-1 gene. |
| 关 键 词： | 鼠 GABA 转运蛋白基因 GAT－1 上行递质 5‘近端序列特征 克隆 表达调节 |
Characterization of 5'- proximal sequence of mouse GABA transporter gene (GAT-1) |
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| FEI JIAN, FANG HUANG, YIN HUA MA, LI HE GUO. Characterization of 5'- proximal sequence of mouse GABA transporter gene (GAT-1)[J]. Cell research, 1997, 7(1): 61-67 | |
| Authors: | FEI JIAN FANG HUANG YIN HUA MA LI HE GUO |
| Abstract: | The cDNA molecule encoding the mouse GABA transporter gene (GAT-1) was used as probe for selecting GAT-1 gene from mouse genomic library. A positive clone, harboring the whole open reading frame of the GAT-1 protein and designated as MGABAT-G, was fished out from the library, the 5' proximal region and nitron 1 were sequenced and analysed, and low homology was found in the above region between GAT-1 genes from mouse and human except some short conserved sequences. The DNA-protein interactions between DNA fragments containing the conserved sequences in the 5' proximal region and nuclear proteins from different tissues of mouse were studied by means of gel-shift assay, and Southern-Western blot. The results indicate a possible positive-negative regulation mode controlling the expression of the mouse GAT-1 gene. |
| Keywords: | Mouse GABA transporter gene expression regulation cloning |
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