Cloning and characterization of the tiger shrimp lysozyme |
| |
Authors: | Ye Xing Gao Feng-Ying Zheng Qing-Mei Bai Jun-Jie Wang Huan Lao Hai-Hua Jian Qing |
| |
Institution: | (1) Key Laboratory of Tropical & Subtropical FishBreeding & Cultivation of CAFS, Pearl River Fisheries Research Institute, Xing Yu Road, Guangzhou, 510380, China;(2) Biology Department, Jia Ying College, Mei Zhou, 514015, China;(3) Fishery College of Guangdong Ocean University, Zhanjiang, 524025, China |
| |
Abstract: | Lysozymes are key proteins to invertebrates in the innate immune responses against bacterial infections. A lysozyme gene isolated
from tiger shrimp, Penaeus monodon, was cloned, sequenced and characterized. The cDNA consists of a signal peptide of 18 amino acids and a mature peptide of
140 amino acids. The lysozyme is presumed to be a chicken-type lysozyme for it possesses two catalytic sites and eight cysteine
residues which are highly conserved across species of chicken-type lysozymes. The lysozyme cDNAs of Penaeus semisulcatus, Litopenaeus vannamei, Macrobrachium nipponense and Macrobrachium rosenbergii were also cloned. High similarities existed among shrimp and prawn lysozymes but phylogenetic relationship of shrimps and
prawns based on lysozyme molecules did not quite consistent with traditional taxonomic classification. High mRNA expression
was detected in hepatopancreas, haemocytes and gill of tiger shrimp. Recombinant lysozyme exhibited potent lytic activities
against fish pathogens providing evidence of the involvement of lysozyme in shrimp immunity. |
| |
Keywords: | Penaeus monodon Prawn Lysozyme Prokaryocyte expression Recombinant protein Bacteriolysis |
本文献已被 PubMed SpringerLink 等数据库收录! |
|