The monosynaptic connection between identified neurons in the snail

Monosynaptic connection between two identified neurones was investigated using electrophysiological and morphological methods in preparation of isolated nervous system of the snail Achatina fulica. Intracellular pressure injection of cobalt chloride was used for staining of neuronal branches. Electrophysiologically revealed synaptic connection between two giant neurones was identified to be monosynaptic by morphological methods.