蛋白质芯片技术进展

人类基因组测序工作的完成 ,引起人们对蛋白质组研究的热忱。蛋白质作为生命活动的执行者 ,种类繁多 ,结构复杂 ,并且其活性与空间结构密切相关 ,需要更为先进的技术去研究和探索。近来出现的蛋白质芯片以并行、高通量检测、分析和处理蛋白质样品 ,发展迅速 ,应用前景广泛。介绍蛋白质芯片的种类、蛋白质固定的表面化学以及不同的检测方法 ,简述蛋白质芯片在不同领域的应用 ,并讨论蛋白质芯片目前存在的问题。

Progress of Protein Chip Technology

After the sequence of the Human Genome is completed,more and more attention focuses on proteome.As executor of biological activities,proteins are more complex than DNA,requiring more advance technology to unravel the structure and biological function of proteins.Protein chip as a new technology is established to investigate proteins in a massively parallel way,which develop rapidly and have promising future.Many new developments in manufacture,detection methods and applications of protein chip are reviewed here and existing problems of protein chip are discussed.