Thiophene accumulation in relation to morphology in roots of Tagetes patula |
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Authors: | A F Croes A J R van den Berg M Bosveld H Breteler G J Wullems |
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Institution: | (1) NOVAPLANT Cell Biotechnology Group, Department of Experimental Botany, University of Nijmegen, Toernooiveld, NL-6525 ED Nijmegen, The Netherlands;(2) Research Institute Itali, P.O. Box 48, NL-6700 AA Wageningen, The Netherlands;(3) Present address: Center for Agrobiological Research, Bornse Steeg 65, NL-6708 PD Wageningen, The Netherlands |
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Abstract: | Roots of marigold (Tagetes patula L.) accumulate thiophenes, heterocyclic sulfurous compounds with strong biocidal activity. In detached roots cultured in vitro, the thiophene content was 5 mol·(g fresh weight)-1 which is 25-times higher than in roots attached to the plant. In roots derived from tissues transformed by Agrobacterium tumefaciens and A. rhizogenes, the morphology and thiophene content varied with the bacterial strain used. Transformation stimulated the elongation of the root tips and the formation of lateral roots but lowered the thiophene level to 20–50% relative to the concentration in untransformed detached roots. A negative correlation was found between the number of laterals in a root system and the thiophene content. Extensive branching and a decrease in thiophene accumulation was evoked in untransformed roots by indole-3-acetic acid (1–10 mol·l-1) added to the medium. Within the roots, the highest thiophene concentrations were found in the tips. The results indicate that auxin directly or indirectly plays a role in the regulation of the thiophene level in root tips.Abbreviations B5
Gamborg's B5 medium
- IAA
Indole-3-acetic acid |
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Keywords: | Agrobacterium Auxin and thiophene in roots Root culture Root morphology Tagetes Thiophene Transformation |
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