Studies on yeast host strains for heterologous P450 expression |
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| Authors: | Gang Fang K Venkateswarlu Diane E Kelly Steven L Kelly |
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| Institution: | (1) Department of Molecular Biology and Biotechnology Krebs Institute for Biomolecular Research, University of Sheffield, S10 2UH Sheffield, UK;(2) Institute of Molecular Biology and Biotechnology, Faculty of Food-Chemical Engineering, Huaihai University, Lianyungang Jiangsu, P.R. of China |
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| Abstract: | The Saccharomyces cerevisiae strain AH22 was capable of human P4501A1 expression without detectable background of yeast P450, unlike ATCC44773. Repeated backcrossing to AH22 produced a strain allowing transformation by vectors carrying various common selectable markers. Background yeast xenobiotic metabolism was observed only with growth on complex medium. |
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