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Evidence for the Presence of Chlorophyllide b in the Green Alga Scenedesmus obliquus in vivo
Authors:Kiriakos Kotzabasis  Horst Senger
Abstract:Chlorophyllide b could be extracted from the wild type of Scenedesmus obliquus and its pigment mutant C-2A'. Its identity was proved by absorption and fluorescence spectroscopy and by a positive hydroxylamine test. Chlorophyllide b could be transformed into pheophorbide b and methylpheophorbide b. The formation of chlorophyllide b from chlorophyll b by dephytylation with chlorophyllase could be ruled out. The stimulation of chlorophyllide b biosynthesis with o-phenanthroline, as described in the literature, could not be confirmed under physiological conditions.
Keywords:chlorophyllide b  methylpheophorbide b  o-phenanthroline  pheophorbide b  Scenedesmus obliquus
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