| 番茄青枯病拮抗菌筛选鉴定及其发酵条件初探 |
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| 引用本文： | 黄明媛,顾文杰,张发宝,徐培智,杨少海,王立群,解开治.番茄青枯病拮抗菌筛选鉴定及其发酵条件初探[J].微生物学通报,2011,38(2):214-220. |
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| 作者姓名： | 黄明媛 顾文杰 张发宝 徐培智 杨少海 王立群 解开治 |
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| 作者单位： | 1. 东北农业大学生命科学学院,黑龙江哈尔滨,150030
2. 广东省农业科学院土壤肥料研究所,广东广州,510640 |
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| 基金项目： | 广东省科技计划重点项目(No. 2009A020101003) |
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| 摘 要： | 从健康番茄根系采样,筛选出4株对番茄青枯病有较强拮抗作用的菌株,在NA培养基上抑菌圈直径>9 mm。其中拮抗菌株YB6抑菌活性最强且拮抗效果稳定,通过形态学观察及部分生理生化特征测定,初步确定为节杆菌属。通过单因素试验进行了发酵条件初步研究,得到适宜的发酵条件为:发酵时间3 d,培养温度30°C,初始pH值9.0,接种量3%,转速100 r/min,碳源蔗糖,氮源酵母浸膏。通过初步优化后拮抗菌株抑菌活性明显增强,最终对青枯病菌SST-Y和G2M1.70抑菌圈直径与NB培养基相比增加了76.72%和81.14%,差异显著。
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| 关 键 词： | 番茄青枯病 拮抗菌株 生理生化 发酵条件 抑菌活性 |
| 收稿时间： | 2010/7/22 0:00:00 |
| 修稿时间： | 2010/11/23 0:00:00 |
Identification and fermentation of antagonistic bacterium against Ralstonia solanacearum |
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| HUANG Ming-Yuan,GU Wen-Jie,ZHANG Fa-Bao,XU Pei-Zhi,YANG Shao-Hai,WANG Li-Qun and XIE Kai-Zhi.Identification and fermentation of antagonistic bacterium against Ralstonia solanacearum[J].Microbiology,2011,38(2):214-220. |
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| Authors: | HUANG Ming-Yuan GU Wen-Jie ZHANG Fa-Bao XU Pei-Zhi YANG Shao-Hai WANG Li-Qun and XIE Kai-Zhi |
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| Institution: | College of life Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China;Soil and Fertilizer Institute, Guangdong Academy of Agricultural Science, Guangzhou, Guangdong 510640, China;Soil and Fertilizer Institute, Guangdong Academy of Agricultural Science, Guangzhou, Guangdong 510640, China;Soil and Fertilizer Institute, Guangdong Academy of Agricultural Science, Guangzhou, Guangdong 510640, China;Soil and Fertilizer Institute, Guangdong Academy of Agricultural Science, Guangzhou, Guangdong 510640, China;College of life Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China;Soil and Fertilizer Institute, Guangdong Academy of Agricultural Science, Guangzhou, Guangdong 510640, China |
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| Abstract: | The objective of this study was to screen and identify antagonistic bacteria against Ralstonia solanacearum from tomato rhizosphere soil. Four strains (YB1, YB6, YB22, YB70) showed strong antagonistic activity, with diameters of inhibition zone above 9 mm. The strain YB6, identified as Arthrobacter sp. according to its morphological, physiological and biochemical characteristics, presented the best and most stable effect against R. solanacearum. The fermentation conditions of the strain YB6 were 30 °C, pH 9.0, inoculum 3%, shaking at 100 r/min, sucrose as carbon source, and yeast condensate as nitrogen source. Under the optimized fermentation conditions, diameters of inhibition zone produced by the strain YB6 against R. solanacearum strains SST-Y and G2M1.70 increased by 76.72% and 81.14%, respectively. |
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| Keywords: | Ralstonia solanacearum Antagonistic bacteria Physiological and biochemical characteristics Fermentation condition Antibacterial activity |
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