裂蹄木层孔菌子实体水提物诱导HepG2细胞凋亡的初步研究

研究裂蹄木层孔菌子实体水提物(WEPL)对人类克隆肝癌细胞系HepG2生长的作用。用裂蹄木层孔菌子实体水提物处理HepG2细胞后,噻唑蓝法(MTT法)可见浓度和时间依赖性抑制细胞增殖;电镜下观察凋亡小体的出现,流式细胞仪技术显示Annexin-Ⅴ染色呈阳性,都证明了HepG2细胞发生了凋亡。RT-PCR和Western Blot分析证实WEPL刺激Bax表达量上调、Bcl-2表达量下调进而诱导了细胞凋亡。结果表明WEPL诱发的克隆人类肝癌细胞系HepG2的细胞凋亡可能是通过上调Bax、下调Bcl-2活性来实现的。

Water extract of Phellinus linteus fruiting body induces apoptosis of HepG2 cells

The effect of water extracts of Phellinus linteus fruit bodies (WEPL) on the growth of HepG2 human colon cancer cells was investigated. Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay showed that the inhibition ratio of HepG2 cells treated with WEPL increased dose-and time-dependently. Microscopic observation proved the occurrence of apoptotic bodies. Flow cytometry detection showed the cells were positively stained by Annexin-Ⅴ. These were the hallmark features of apoptosis. The mRNA expression level and protein level of Bax were adjusted up while that of Bcl-2 were adjusted down. It seems possible that WEPL induces HepG2 cell apoptosis through down-regulating the expression of Bcl-2 gene and up-regulating the expression of Bax gene.