大熊猫精子获能和顶体反应过程中钙分布变化规律的研究

应用焦锑酸钾原位定位法对大熊猫精子获能和顶体反应过程中进行钙定位研究，发现未获能精子的 Ｃａ２＋主要结合于顶体前区和赤道段质膜外侧和顶体内膜内侧（核膜侧）；随着获能的进行，Ｃａ２＋进入精子内部并主要结合于顶体区质膜内侧和顶体外膜外侧；顶体反应的精子，Ｃａ２＋结合于顶体内膜外侧、顶体后区质膜外侧和分散存在于释放的顶体内容物中，有些顶体反应精子的顶体内膜外侧结合的Ｃａ２＋特别丰富。精子尾部的Ｃａ２＋主要分布于中段线粒体内，且其内所含Ｃａ２＋含量随着获能和顶体反应而增加。另外尾部致密纤维和轴丝处也有少量Ｃａ２＋分布。

CHANGES OF CALCIUM DISTRIBUTION DURING CAPACITATION AND ACROSOME REACTION OF THE GIANT PANDA SPERM

Calcium was localized by in situ precipitation with potassium antimonate in giant panda sperm during capacitation and acrosome reaction. Before capacitation the calcium in sperm head was mainly l.localized on the outer surface of plasma membrane (PM) of the periacrosomal region. There wasalso a little calcium on the inner surface of the inner acrosomal membrane (IAM). During capacitation calcium was concentrated on the outer surface of the outer acrosomal membrane (OAM) and on the inner surface of PM of the periacrosomal region. During acrosome reaction calcium bound to the IAM and existed among the released acroso- mal contents. There were also some fine granules of precipitate on the outer surface of PM of the post acrosomal region during acrosome reaction. Calcium in sperm tail was mainly distributed in the matrix of mitochondria of the midpiece and the amount of calcium in mitochondria increased along with the capacitation process. The pyroantimonate precipitate granules were also distributed in the matrix near the outer dense fibres and near the axial filament complex.