| 林木病原菌广谱拮抗菌株的筛选及功能研究 |
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| 作者姓名： | 刘彩霞 焦如珍 赵京京 |
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| 作者单位： | 1.中国林业科学研究院林业研究所, 北京 100091;
2.湖南省林业科学院, 长沙 410004 |
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| 基金项目： | 国家重点研发计划项目（2016YFD0600302）资助。 |
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| 摘 要： | 以尖孢镰刀菌、腐皮镰孢菌、胶孢炭疽菌、拟盘多毛孢菌等林木常见病原菌为指示菌，用平板对峙法和菌丝球干重法，从土壤固氮细菌群中筛选广谱拮抗菌株，并调查了各菌株的溶磷及解钾能力。结果表明：对4种病原菌拮抗能力较强的菌株有24株，其中11株能溶解无机磷，7株能溶解有机磷，4株有解钾功能。通过拮抗能力复筛得到最优菌株为菌株2024和7014。菌种鉴定结果表明2024菌株为枯草芽孢杆菌亚种（Bacillus subtilis subsp. subtilis），7014菌株为成团泛菌（Pantoea vagans）。
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| 关 键 词： | 林木 病原菌 拮抗作用 溶磷 解钾 |
| 收稿时间： | 2017-08-10 |
Screening and Functional Research of Broad Spectrum Antagonistic Strains Against Forest Pathogenic Fungi |
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| Authors: | LIU Caixia JIAO Ruzhen ZHAO Jingjing |
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| Institution: | 1.Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China;
2.Hunan Academy of Forestry, Changsha 410004, China |
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| Abstract: | Broad-spectrum antagonistic bacterium against forest pathogenic fungi, such as Fusarium oxysporum Schltdl, Haematonectria haematococca (Berk. & Broome) Samuels & Rossman, Colletotrichum gloeosporiodes Penz, Pestalotiopsisapiculatus (Huang.) Huang, were screened by plate confrontation method and mycelial dry weight method from nitrogen fixing bacteria in soil. The ability of phosphorus solubilization and potassium dissolving of strains were also studied. The results showed that there were 24 strains had strong antagonistic ability to 4 pathogenic bacterium, 11 strains of dissolved inorganic phosphorus, 7 strains of dissolved organic phosphorus and 4 strains of releasing potassium. The results of strain identification showed that strain 2024 was Bacillus subtilis subsp. subtilis and the strain 7014 was Pantoea vagans. |
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| Keywords: | forest pathogenic fungi antagonism phosphate solubilization release potassium |
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