| Abstract: | The effects of osmotic pressure on inward sodium current during a change in temperature were investigated during experiments on isolated rat spinal ganglia neurons using techniques of intracellular perfusion and voltage clamping. It was found that the effect of osmotic pressure on the kinetic parameters of sodium current does not depend on temperature over a wide range of 8–40°C; the apparent values of activation energies for the activation and inactivation processes do not dependent on degree of osmolality. Overall findings would appear to indicate that the osmotic pressure effect is actually initiated by association with aqueous transmembrane flux. Opinions are expressed as to the location of the structures through which this aqueous flux passes and of the sodium channel gating mechanism, together which the molecular mechanisms of interaction between these two elements.A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 18, No. 4, pp. 518–525, July–August, 1986. |
