Mutagenesis at the ad-3A and ad-3B loci haploid UV-sensitive strains of Neurospora crassa. III. Comparison of dose-response curves for inactivation and mutation induced by gamma-rays |
| |
| Authors: | M E Schüpbach F J de Serres |
| |
| Institution: | 1. Laboratory of Genetics, Department of Regulation Biology, Faculty of Science, Saitama University, Urawa, 338 Japan;2. University of Tennessee, Department of Veterinary Medicine, Knoxville, TN, U.S.A.;3. The Queen Elizabeth Hospital, Cytogenetics Unit, Woodville, South Australia 5011, Australia;4. Office of the Associate Director for Genetics, National Institute of Environmental Health Science, Research Triangle Park, NC 27709, U.S.A. |
| |
| Abstract: | Gamma-Ray-induced inactivation and induction of mutations at the ad-3A and ad-3B loci of Neurospora crassa have been compared among 6 different UV-sensitive strains and a standard wild-type strain. The 6 strains show varying degrees of sensitivity to gamma-ray-induced inactivation, with the relative sensitivity at 37% survival being uvs-6 greater than upr-1 greater than uvs-2 greater than uvs-3 greater than wild-type greater than uvs-5 greater than uvs-4. Studies on the induction of ad-3 mutants by gamma-rays show that when the dose-response curve (expressed in terms of ad-3 mutants among the surviving colonies) of the UV-sensitive strains are compared with wild-type, the 2 excision-repair-deficient mutants uvs-2 and upr-1 exhibit enhanced ad-3 mutant frequencies, uvs-3 exhibits reduced ad-3 mutant frequencies whereas both uvs-4 and uvs-5 show lower mutant frequencies than wild-type. |
| |
| Keywords: | MMS methyl methanesulfonate |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
