Isolation of a complementing activity for a dna-B mutant |
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Authors: | M Koiyama E Milewski |
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Institution: | Institut de Biologie Moléculaire Université Paris VII 75005 Paris - France |
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Abstract: | A cell free extract which displays temperature sensitive DNA synthesis in the presence of single strand DNA and ATP was prepared from a mutant. Following an activity which would reverse this temperature sensitivity, a protein fraction was isolated. The absence of this fraction in a mutant indicates that this protein corresponds to the product. |
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