Paracrine effects of oocyte secreted factors and stem cell factor on porcine granulosa and theca cells <Emphasis Type="Italic">in vitro</Emphasis> |
| |
Authors: | Email author" target="_blank">Victoria?BrankinEmail author Marcus?RP?Mitchell Bob?Webb Morag?G?Hunter |
| |
Institution: | (1) School of Biosciences, University of Nottingham, Sutton Bonington Campus, LE12 5RD Loughborough, Leicestershire, UK |
| |
Abstract: | Oocyte control of granulosa and theca cell function may be mediated by several growth factors via a local feedback loop(s)
between these cell types. This study examined both the role of oocyte-secreted factors on granulosa and thecal cells, cultured
independently and in co-culture, and the effect of stem cell factor (SCF); a granulosa cell derived peptide that appears to
have multiple roles in follicle development. Granulosa and theca cells were isolated from 2–6 mm healthy follicles of mature
porcine ovaries and cultured under serum-free conditions, supplemented with: 100 ng/ml LR3 IGF-1, 10 ng/ml insulin, 100 ng/ml
testosterone, 0–10 ng/ml SCF, 1 ng/ml FSH (granulosa), 0.01 ng/ml LH (theca) or 1 ng/ml FSH and 0.01 ng/ml LH (co-culture)
and with/without oocyte conditioned medium (OCM) or 5 oocytes. Cells were cultured in 96 well plates for 144 h, after which
viable cell numbers were determined. Medium was replaced every 48 h and spent medium analysed for steroids. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|