Micropilot: automation of fluorescence microscopy-based imaging for systems biology |
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| Authors: | Conrad Christian Wünsche Annelie Tan Tze Heng Bulkescher Jutta Sieckmann Frank Verissimo Fatima Edelstein Arthur Walter Thomas Liebel Urban Pepperkok Rainer Ellenberg Jan |
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| Institution: | Advanced Light Microscopy Facility, European Molecular Biology Laboratory, Heidelberg, Germany. |
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| Abstract: | Quantitative microscopy relies on imaging of large cell numbers but is often hampered by time-consuming manual selection of specific cells. The 'Micropilot' software automatically detects cells of interest and launches complex imaging experiments including three-dimensional multicolor time-lapse or fluorescence recovery after photobleaching in live cells. In three independent experimental setups this allowed us to statistically analyze biological processes in detail and is thus a powerful tool for systems biology. |
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