Selection for salt tolerance in vitro using microspore-derived embryos ofBrassica napus cv. topas,and the characterization of putative tolerant plants |
| |
Authors: | M Hafizur Rahman S Krishnaraj Trevor A Thorpe |
| |
Institution: | (1) Plant Physiology Research Group, Department of Biological Sciences, University of Calgary, T2N 1N4 Calgary, Alberta, Canada |
| |
Abstract: | Microspore-derived embryos ofBrassica napus cv. Topas that survived salt stress, were obtained after selection against otherwise lethal doses (0.6 and 0.7%) of NaCl
after mutagen treatment. A total of 10 salt-surviving embryos were obtained out of a possible 834 000 embryos that were mutagenized.
One embryo out of a possible 845 000 obtained from nonmutagenized controls survived but failed to develop into a plant. Visual
assessment after salt stress indicated that both the putative salt-tolerant plants and plants from control seeds behaved similarly.
However, based on individual characteristics related to salt tolerance, one of the lines (PST-2) accumulated less sodium and
retained more potassium, and hence was able to maintain a more favorable Na:K ratio as compared to the controls under salt
stress. Also chlorophylla fluorescence induction and quenching signals indicated a high energetic state of the thylakoid membranes in PST-2 under salt
stress. The other putative salt-tolerant line (PST-1) had a higher background level of proline that may have enabled it to
survive salt stress during initial screening, although its later performance was no better than the control plants. |
| |
Keywords: | Brassica napus microspore-derived embryos haploid salt-tolerance selection mutagenesis proline ions (sodium potassium) chlorophylla fluorescence |
本文献已被 SpringerLink 等数据库收录! |
|