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Toxoplasma gondii: Serological recognition of reinfection
Authors:Dzitko Katarzyna  Staczek Pawel  Gatkowska Justyna  Dlugonska Henryka
Affiliation:Department of Immunoparasitology, University of Lodz, Poland. dzika@biol.uni.lodz.pl
Abstract:
Using murine chronic toxoplasmosis as an experimental model, we examined the utility of immunoenzymatic methods in recognizing reinfection in chronically infected individuals. Primary infection with avirulent Toxoplasma gondii DX strain (genotype II) induced strong immunity protecting the mice from mortality after inoculation with LD(100) of virulent BK strain (genotype I) and triggered highly expressed antibody production, within one new isotype detected by comparative immunoblots. The parasites multiplying at the site of reinfection were of BK origin as found by RAPD-PCR. The results revealed that the immunoblot assay seems to be a useful and reliable method for the monitoring of specific antibody profile in chronically infected individuals. In our opinion ELISA combined with immunoblot could enable the recognition of reinfection cases in humans, but earlier our experimental data should be verified in clinical laboratory.
Keywords:Toxoplasma gondii   Reinfection   ELISA   Immunoblot   RAPD-PCR
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