Transformation of mouse BALB/c 3T3 cells with human basic fibroblast growth factor cDNA. |
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| Authors: | R Sasada T Kurokawa M Iwane and K Igarashi |
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| Institution: | Central Research Division, Takeda Chemical Industries, Ltd., Osaka, Japan. |
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| Abstract: | The expression of human basic fibroblast growth factor (bFGF) cDNA in mouse BALB/c 3T3 clone A31 cells induced morphological transformation. These transformed cells grew well and reached more than a sixfold-higher saturation density than parental A31 cells even in serum-free medium. They were able to form colonies in soft agar. The phenotypic alteration in the transformed cells was reversed by the addition of anti-human bFGF antibodies to the medium. These results suggest that the cellular transformation mediated by bFGF is caused by autocrine stimulation with secreted bFGF molecules. |
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