Protein-free medium for C-1300 mouse neuroblastoma cells |
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Authors: | Peter C Agy Gary D Shipley Richard G Ham |
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Institution: | (1) Department of Molecular, Cellular and Developmental Biology, University of Colorado, 80309 Boulder, Colorado;(2) Present address: Latter Day Saints Hospital, Salt Lake City, Utah |
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Abstract: | Summary An optimized medium, designated MCDB 411, has been developed for mouse neuroblastoma cells. At cell densities of 1×104 cells/cm2 or greater, several different clones of C1300 mouse neuroblastoma cells can be cultured serially in Medium MCDB 411 with
no serum or other undefined supplementation and with a doubling time of about 24 h. At clonal densities it is necessary to
supplement the medium with 1.0 μg/ml insulin. Alternately, good clonal growth can be obtained without direct supplementation
by coating the culture dishes with insulin and rinsing off all that is not tightly bound. Primary cultures of cells from serially
transplanted C1300 tumors that have never been cultured previously in vitro can be established directly in unsupplemented
Medium MCBD 411 with rapid initiation of multiplication and no apparent crisis or selection for minority cell types. The availability
of a synthetic medium that supports growth of neuroblastoma cells without supplementation should facilitate the use of these
cells as model systems for the study of neuronal function and differentiation.
This work was supported by Grant CA-15305 from the National Cancer Institute. |
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Keywords: | neuroblastoma cells protein-free growth MCDB 411 insulin clonal growth |
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