Characteristics of pullulanases from extremely thermophilic archaea isolated from deep-sea hydrothermal vents |
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| Authors: | Hubert Gantelet Christine Ladrat Anne Godfroy Georges Barbier Francis Duchiron |
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| Institution: | (1) Laboratoire de Microbiologie Industrielle, Université de Reims-Champagne-Ardenne, UFR Sciences Exactes et Naturelles, Moulin de la Housse, Reims, France;(2) IFREMER, Centre de Brest, Laboratoire de Biotechnologie des Micro-organismes Hydrothermaux, Plouzané, France |
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| Abstract: | Two out of three extremely thermophilic anaerobic archaea, isolated from deep-sea hydrothermal vents, produced pullulanase activity in the presence of maltose in the growth medium. Enzyme activities were mainly extracellular and characterized by optimum temperatures of 95°C and 80–95°C, optimum pH of 5.0–7.0 and a high degree of thermostability. One strain when grown in a fermenter with maltose as inducer produced pullulanase at 35 U/l. © Rapid Science Ltd. 1998 |
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| Keywords: | pullulanase deep-sea hydrothermal vents Thermococcus thermostability archaea |
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