Kinesin-1 plays a role in transport of SNAP-25 to the plasma membrane |
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| Authors: | April M. Morton |
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| Affiliation: | Centre for Virus Research, Westmead Millennium Institute, The University of Sydney and Westmead Hospital, Westmead, NSW 2145, Australia |
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| Abstract: | The cellular molecular motor kinesin-1 mediates the microtubule-dependent transport of a range of cargo. We have previously identified an interaction between the cargo-binding domain of kinesin-1 heavy chain KIF5B and the membrane-associated SNARE proteins SNAP-25 and SNAP-23. In this study we further defined the minimal SNAP-25 binding domain in KIF5B to residues 874-894. Overexpression of a fragment of KIF5B (residues 594-910) resulted in significant colocalization with SNAP-25 with resulting blockage of the trafficking of SNAP-25 to the periphery of cells. This indicates that kinesin-1 facilitates the transport of SNAP-25 containing vesicles as a prerequisite to SNAP-25 driven membrane fusion events. |
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| Keywords: | GRIP1, α-amino-3-hydroxy-5-methylisoxazole-4-propionate receptor subunit-GluR2-interacting protein HSV-1, herpes simplex virus type 1 KHC, kinesin-1 heavy chain KIF, kinesin superfamily KLC, kinesin-1 light chain RanBP2, Ran-binding protein 2 SNAP-23, synaptosomal-associated protein (SNAP) of 23 kDa SNAP-25, SNAP of 25 kDa SNARE, N-ethylmaleimide-sensitive factor attachment protein receptor TPR, tetratricopeptide repeat |
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