IL-11 expression in retinal and corneal cells is regulated by interferon-γ |
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| Authors: | Chandrasekharam N Nagineni Abitha William Barbara Detrick |
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| Institution: | a Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, MD, United States
b Department of Pathology, Johns Hopkins University, School of Medicine, Baltimore, MD, United States |
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| Abstract: | Interleukin-11 (IL-11) is an anti-apoptotic, anti-inflammatory cytokine with hematopoietic potential. The expression and protective actions of IL-11 have not been explored in the eye. The expression of IL-11 in primary cultures of human retinal pigment epithelial (HRPE) and human corneal fibroblast (HCRF) cells were evaluated in these studies. Constitutive secretion of IL-11 was not observed in either HRPE or HCRF. TNF-α + IL-1 induced IL-11 secretion and this production was inhibited by NFκB pathway inhibitors. IFN-γ significantly inhibited TNF-α and IL-1 induced IL-11 secretion and inhibitors of JAK-STAT pathway reversed this inhibition. TGF-β induced IL-11 secretion that was blocked by TGF-β receptor 1 inhibitor but not by IFN-γ. RT-PCR analysis confirmed the effects of IL-1, TNF-α, IFN-γ and TGF-β on IL-11 secretion at mRNA levels. Our results demonstrate that IL-11 is dramatically up regulated in retina and cornea cells and that IFN-γ is a physiological inhibitor of IL-11 expression. |
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| Keywords: | IL-11 IFN-γ TNF-α IL-1 TGF-β Retina Cornea |
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