Physiological variations in plasma testosterone in normal adult male subjects measured by a competitive protein-binding technique |
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| Authors: | M J Thomas R D Gordon J R Smid |
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| Affiliation: | 1. Department of Psychology, University of Oregon, 1227 University of Oregon, Eugene, OR 97403, USA;2. Department of Management & Organization, NUS Business School, National University of Singapore, Mochtar Riady Building, 15 Kent Ridge Drive, Singapore 119245, Republic of Singapore;3. International Institute of Management Development (IMD), Chemin de Bellerive 23, 1003 Lausanne, Switzerland;4. Saw Swee Hock School of Public Health, National University of Singapore, Tahir Foundation Building, 12 Science Drive 2, Singapore 117549, Republic of Singapore;5. PAPRSB Institute of Health Sciences, Universiti Brunei Darussalam, Jalan Tungku Link Gadong, BE1410 Negara, Brunei Darussalam |
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| Abstract: | A method of estimating plasma testosterone utilizing a single paper chromatographic procedure and competitive protein-binding is described. The influence of some conditions of sample collection on plasma testosterone has been investigated. Delay in the separation of plasma from cells and the type of anticoagulant used were both without significant effect. There was an approx. two-fold variability in plasma testosterone concn. in samples withdrawn daily, weekly and monthly from four young, normal adult males. |
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