| N^＋注入Lactobacillus bulgarius诱变选育高产ACE抑制剂的研究 |
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| 引用本文： | 马丽丽,王志耕,陈美姣,王玲琴.N^＋注入Lactobacillus bulgarius诱变选育高产ACE抑制剂的研究[J].激光生物学报,2010,19(3):391-396. |
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| 作者姓名： | 马丽丽 王志耕 陈美姣 王玲琴 |
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| 作者单位： | 1. 安徽农业大学动物科技学院,安徽,合肥,230036;安徽省乳品工程技术研究中心,安徽,合肥,230036
2. 安徽省乳品工程技术研究中心,安徽,合肥,230036 |
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| 摘 要： | 采用N^＋注入技术对实验室保存的4株保加利亚杆菌L601、L602、L603、L606进行诱变处理，旨在选育出高产血管紧张素转换酶（angiotensin converting enzyme，ACE）抑制剂菌株。结果表明，在离子注入能量为20keV，剂量为3．0×0．5×10^15 ions／cm^2条件下，诱变效果较好。从正突变菌株中反复筛选，获得1株对ACE抑制活性较高的菌株M602，其ACE抑制率为82．49％。经过培养条件优化，其对ACE抑制率进一步提高为85．12％，经连续传代实验，其性状遗传稳定。
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| 关 键 词： | 保加利亚杆菌 氮离子 ACE |
A Study on High-yielding Strains of ACE Inhibitory Peptides through the N+ Implanting into Lactobacillus bulgarius |
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| MA Li-li,WANG Zhi-geng,CHEN Mei-jiao,WANG Ling-qing.A Study on High-yielding Strains of ACE Inhibitory Peptides through the N+ Implanting into Lactobacillus bulgarius[J].ACTA Laser Biology Sinica,2010,19(3):391-396. |
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| Authors: | MA Li-li WANG Zhi-geng CHEN Mei-jiao WANG Ling-qing |
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| Institution: | 1. School of Animal Science and Technology, AnHui Agricultural University, Hefei 230036, Anhui,China; 2. Engineering Research Center of Dairy Products Processing in Anhui, Hefei 250036, Anhui, China) |
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| Abstract: | The aim of this paper was to obtain high-yielding strains of ACE inhibitory peptides through the N^＋ implanting into four strains of Lactobacillus bulgarius （L601, L602, L603 and L606）. The results showed that the best mutation condition were that energy is 20 keV, and dose is 3.0 × 0. 5 × 10^15 ions/cm^2. The strains M602 with stronger ACE inhibitory activity was obtained from the mutant strains and its ACE inhibitory rate was 82.49 %. After improving the culture conditions, the ACE inhibitory rate increased to 85.12 % ,and it can be inherited stably. |
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| Keywords: | ACE |
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