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| 光敏核不育水稻花药IAA的免疫组织化学分析 | |
| 引用本文： | 李念华 童哲. 光敏核不育水稻花药IAA的免疫组织化学分析[J]. Acta Botanica Sinica, 2000, 42(10): 1045-1050 |
| 作者姓名： | 李念华 童哲 |
| 作者单位： | 中国科学院植物研究所,北京 |
| 基金项目： | PioneerFundofClimbingProgramofChina (95_2 9_0 3)，TheNationalNaturalScienceFoundationofChina (39570 0 79) |
| 摘 要： | 首次应用免疫组织化学分析方法对长日和短日处理后的农垦５８Ｓ和对照农垦５８（Ｏｒｙｚａ ｓａｔｉｖａ Ｌ．ｓｕｂｓｐ．ｊａｐｏｎｉｃａ）花药中的ＩＡＡ进行了定位研究和相对水平的比较。结果表明，此方法可反映游离态ＩＡＡ在花药中的分布及其相对水平的变化。从雌雄蕊原基形成期至单核晚期的５个时期中，经长日照处理的农垦５８Ｓ花药中的ＩＡＡ水平都低于短日照处理的农垦５８Ｓ及在不同光周期处理下的农垦５８花药。对花 |
| 关 键 词： | 水稻 花药 生长素 光周期 免疫组织化学定位 |
Immunohistochemical Analysis of IAA in Anthers of the Photoperiod-sensitive Genic Male-sterile Rice |
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| LI Nian_Hua,TONG Zhe ,LU Shan_Fa. Immunohistochemical Analysis of IAA in Anthers of the Photoperiod-sensitive Genic Male-sterile Rice[J]. , 2000, 42(10): 1045-1050 | |
| Authors: | LI Nian_Hua TONG Zhe LU Shan_Fa |
| Affiliation: | LI Nian_Hua,TONG Zhe *,LU Shan_Fa |
| Abstract: | The immunohistochemical localization of IAA and the comparison of their relative levels were carried out for the first time in the anthers of Nongken 58S and its wild type Nongken 58 (Oryza sativa subsp. japonica) after long_day and short_day treatments. The distribution of free_IAA in anthers and its dynamic variation could be reflected by this method. The results showed that the IAA level in the anthers of Nongken 58S after long_day treatment was much lower than that in short_day_treated Nongken 58S and those in wild type Nongken 58 in five stages from pistil and stamen primordia formation to late uninucleate stage. The possible reasons for IAA deficiency in Nongken 58S_LD anthers and its relationship with fertility alteration were also discussed. |
| Keywords: | rice anther auxin photoperiod male sterility immunohistochemical localization |
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