Genotyping Echinococcus granulosus from dogs from Western Iran |
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| Authors: | Farzad Parsa Majid Fasihi Harandi Sima Rostami Mitra Sharbatkhori |
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| Institution: | 1. Department of Laboratory Sciences, Faculty of Para-Medicine, Islamic Azad University, Borujerd Branch, Borujerd, Iran;2. Zoonoses Research Center, Department of Parasitology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran;3. Laboratory Science Research Center, Golestan University of Medical Sciences, Gorgan, Iran;4. Department of Parasitology and Mycology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran;1. Faculty of Veterinary Science, Centre for Animal Biotechnology, The University of Melbourne, Parkville, Victoria 3010, Australia;2. Parasitology Unit, University of Hohenheim, 70599 Stuttgart, Germany;3. Faculty of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3010, Australia;1. Swiss Tropical and Public Health Institute, Socinstrasse 57, P.O. Box, CH-4002 Basel, Switzerland;2. University of Basel, Petersplatz 1, CH-4003 Basel, Switzerland;3. Laboratoire Central Vétérinaire, Km 8, Route de Koulikoro, BP 2295 Bamako, Mali;4. Institute of Parasitology, Vetsuisse-Faculty, University of Zurich, Winterthurerstrasse 266a, CH-8057 Zürich, Switzerland;1. Department of Veterinary Parasitology, Islamic Azad University Babol-Branch, Iran;2. Department of Cellular and Molecular, Islamic Azad University Babol-Branch, Iran;3. Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran;4. Department of Parasitology and Mycology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran;5. Departement of Epidemiology, Pasteur Institute of Tehran, Tehran, Iran;1. Department of Parasitology, Asahikawa Medical University, Asahikawa, Midorigaoka-Higashi 2-1, Hokkaido 078, Japan;2. Infection Biology Program/Department of Bacteriology and Immunology, Haartman Institute, P.O. Box 21, FI-00014 University of Helsinki, Finland |
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| Abstract: | Cystic echinococcosis is a zoonotic infection caused by the dog tapeworm, Echinococcus granulosus. In the present study, adults of E. granulosus (n = 20) were collected from 71 dogs from Western Iran and were genetically characterized using DNA sequencing of the partial mitochondrial cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase 1 (nad1). Consensus sequences were obtained for cox1 (366) and nad1 (471) genes. Phylogenetic analysis of concatenated nad1 and cox1 nucleotide sequence data was performed using Bayesian Inference approach. Overall, the dog isolates indicated nine different sequences in cox1 and seven in nad1 genes. Three genotypes (G1 75%], G2 10%] and G3 15%]) were identified from the isolates. The G2 sequences indicated 100% homology with reference G2 sequence in both cox1 (Genbank accession number M84662) and nad1 (AJ237633) genes. G3 sequences showed 100% homology with G3 reference sequence in nad1 (AJ237633), but displayed two different cox1 profiles, each having 99% homology with reference G3 sequence (M84663). In the phylogenetic tree all of the isolates were grouped into a distinct cluster corresponding to the G1–G3 complex with relevant reference sequences. The presence of G1 genotype (sheep strain) of E. granulosus sensu stricto as dominant genotype in dogs is emphasized. To the best of our knowledge, this study established the first record of E. granulosus sensu stricto, G2 genotype in Iran. |
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