| PCR产物的RFLP分析在黄芪亚族（豆科）系统学研究中的应用初探 |
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| 引用本文： | 丁士友,顾红雅.PCR产物的RFLP分析在黄芪亚族（豆科）系统学研究中的应用初探[J].Acta Botanica Sinica,1995,37(2):97-102. |
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| 作者姓名： | 丁士友 顾红雅 |
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| 作者单位： | 中国科学院昆明植物研究所!昆明650204(丁士友)，北京大学蛋白质工程及植物基因工程国家重点实验室!北京100871(顾红雅，瞿礼嘉，陈章良) |
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| 摘 要： | 用聚合酶链式反应（ＰＣＲ）将豆科黄芪亚族７属９种及甘草亚族１属１种植物叶绿体基因组中ｎｄｈＦ和ｐｓｂＡ基因中一段约３．１ｋｂ的ＤＮＡ扩增出来，并摸索出一最佳的ＰＣＲ条件，使得此条带得以特异性扩增，可直接用于限制性内切酶水解。通过对此扩增片段的限制性片段长度多态性（ＲＦＬＰ）的初步分析，认为利用ＰＣＲ扩增出的ＤＮＡ进行ＲＦＬＰ分析来探讨植物的系统进化问题在中国现行条件下大有潜力，其方法简单易行，结果
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| 关 键 词： | 黄芪亚族 聚合酶链反应 RFLP 豆科 |
A PRELIMINARY STUDY ON THE USE OF RFLP ANALYSIS OF THE PCR AMPLIFIED PRODUCTS IN THE SYS TEMATIC INVESTIGATION OF THE SUBTRIBE ASTRAGALINAE (FABACEAE) |
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| Authors: | Ding Shi you |
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| Abstract: | The analysis of variation at the molecular level has been proven extremely useful in the reconstruction of plant phylogenetic relationships.A DNA fragment of 3100 base pairs (bp) in the chloroplast genes ndhF and psbA has been amplified from 9 species of 7 genera in the subtribe Astragalinae and 1 species in the subtribe Glycyrrhizinae.A proper procedure for specifically PCR amplifing the 3 1 kb fragment was presented.By this procedure, the PCR products could be digested directly after amplification.The restriction fragment length polymorphism (RFLP) analysis of the PCR amplified products indicated that it had potential systematic significances in the phylogenetic studies of the subtribe Astragalinae.This approach could also reduce time, expense and the amount of DNA required, and furthermore, obtain reliable results. |
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| Keywords: | Astragalinae Polymerase chain reaction Restriction frugment length polymorphism |
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