Specificity of Hpa II and Hae III DNA methylases |
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| Authors: | Michael B Mann and Hamilton O Smith |
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| Institution: | Department of Microbiology, The Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA |
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| Abstract: | The methylases M.HaeIII and M.HpaII recognize the tetranucleotide sequences Formula: see text] and Formula: see text] respectively, in DNA, and transfer a methyl group from S-adenosylmethionine to the 5-position of cytosine on each strand as indicated by the asterisks. Restriction endonuclease R.HaeIII does not cleave the methylated sequence Formula: see text] but can cleave Formula: see text] in which methylation is introduced on the unnatural external cytosine positions. Similarly, R.HpaII does not cleave Formula: see text] but can cleave Formula: see text].Images |
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