Low-SDS Blue native PAGE |
| |
Authors: | Klodmann Jennifer Lewejohann Dagmar Braun Hans-Peter |
| |
Institution: | Institute for Plant Genetics, Faculty of Natural Sciences, Leibniz Universit?t Hannover, Hannover, Germany. |
| |
Abstract: | SDS normally is strictly avoided during Blue native (BN) PAGE because it leads to disassembly of protein complexes and unfolding of proteins. Here, we report a modified BN-PAGE procedure, which is based on low-SDS treatment of biological samples prior to native gel electrophoresis. Using mitochondrial OXPHOS complexes from Arabidopsis as a model system, low SDS concentrations are shown to partially dissect protein complexes in a very defined and reproducible way. If combined with 2-D BN/SDS-PAGE, generated subcomplexes and their subunits can be systematically investigated, allowing insights into the internal architecture of protein complexes. Furthermore, a 3-D BN/low-SDS BN/SDS-PAGE system is introduced to facilitate structural analysis of individual protein complexes without their previous purification. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|