Proteomic Changes in Newly Synthesized <Emphasis Type="Italic">Brassica napus</Emphasis> Allotetraploids and Their Early Generations |
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Authors: | Fang Kong Shanjing Mao Jinjin Jiang Juan Wang Xiaoping Fang Youping Wang |
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Institution: | (1) College of Bioscience and Biotechnology, Yangzhou University, Yangzhou, 225009, China;(2) Academy of Biological and Chemical Engineering, Anhui Polytechnic University, Wuhu, 24100, China;(3) Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, 430062, China; |
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Abstract: | Polyploidy is a prominent process in higher plants and is often described as a genomic shock that may induce stress and defense
responses. The Brassica napus allotetraploid model was chosen to investigate the proteomic modifications that occur during allopolyploid formation. Large-scale
analysis of the proteome from the leaves of B. napus was performed and compared with the homozygous diploid progenitors, Brassica rapa and Brassica oleracea, and among the proteomic changes in B. napus in the early generations (F1–F4). The abundance of all these differentially expressed proteins in the F1 generation differed from that of the corresponding proteins expressed in its progenitors, some of which relatively deviated
from mid-parent predictions, exhibiting somewhat non-additive expression repatterning. Proteomic changes in the resynthesized
B. napus from the first to the fourth generations were detected, which indicated that gene silencing was a permanent phenomenon and
it could be reactivated at any moment. Although leaf proteins were extensively modified in synthetic B. napus, the distribution of the “housekeeping” proteins was not disturbed. Moreover, no evidence of chaos or large disorder was
observed after the merging of the two genomes. Instead, a novel order quickly developed, which might evolve in further generations
of synthetic B. napus. |
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