| 20#-5菌株代谢产物中抗菌成分的纯化和结构测定 |
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| 引用本文： | 蓝希钳,谢洁,袁志辉,胡军华,肖杰,周泽扬. 20#-5菌株代谢产物中抗菌成分的纯化和结构测定[J]. 微生物学通报, 2005, 32(6): 16-19 |
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| 作者姓名： | 蓝希钳 谢洁 袁志辉 胡军华 肖杰 周泽扬 |
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| 作者单位： | 1. 西南农业大学农业部蚕学重点实验室微生物研究中心,重庆,400716
2. 西南农业大学农业部蚕学重点实验室微生物研究中心,重庆,400716;重庆师范大学生命科学学院,重庆,400047 |
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| 基金项目： | 重庆市科委重大攻关项目(No.7592) |
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| 摘 要： | 为弄清假单胞菌20#-5菌株代谢产物中的抗菌活性成分物质种类,先通过有机溶剂萃取、硅胶柱层析分离和HPLC C-18柱纯化得到纯品,再利用UV、IR、MS、1H-NMR及13C-NMR等光谱分析,确定其中的两个活性成分为phenazine-1-carboxylic ac id和3-n-heptyl-3-hydroxy-1,2,3,4-tetrahydroqu inoline-2,4-d ione。
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| 关 键 词： | 活性成分 纯化 结构测定 |
| 文章编号： | 0253-2654(2005)06-0016-04 |
| 收稿时间： | 2005-01-17 |
| 修稿时间： | 2005-04-17 |
Isolation, Structural Identification of Active Components from Metabolite of Pseudomonas sp. 20#-5 |
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| LAN Xi-Qian,XIE Jie,YUAN Zhi-Hui,FU Jun-Hua,XIAO Jie,ZHOU Ze-Yang. Isolation, Structural Identification of Active Components from Metabolite of Pseudomonas sp. 20#-5[J]. Microbiology China, 2005, 32(6): 16-19 |
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| Authors: | LAN Xi-Qian XIE Jie YUAN Zhi-Hui FU Jun-Hua XIAO Jie ZHOU Ze-Yang |
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| Affiliation: | 1 The Key Sericultural Laboratory of Agricultural Ministry, Southwest Agricultural University, Chongqing 400716 ;2 College of Bioscience of Chongqing Normal University, Chongqing 400047 |
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| Abstract: | Three active components were isolated from the metabolite of Pseudomonas sp.20~(#)-5 by using organic solvent extraction,silica gel column chromatogram and C-18 reverse phase chromatogram in HPLC.Two of them were identified as phenazine-1-carboxylic acid and 3-n-heptyl-3-hydroxy-1,2,3,4-tetrahydroquinoline-2,4-dione respectively. |
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| Keywords: | Active component Purification Structural identification |
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