Abstract: | Hydrocarbons present on the cuticle surface of adult face flies, Musca autumnalis, were identified by GC-MS and quantified by GC. Hydrocarbons consisted of n-, monomethyl, and dimethyl alkanes ranging in chain length from 23–29 carbons. Also present were monounsaturated alkenes with chain lengths of 23, 25, 27, and 29 carbons. Wild-caught flies were extracted and hydrocarbon profiles determined for both diapausing and reproductive adult males and females. Few qualitative differences were found between males and females in the hydrocarbon profile. Differences in percent composition were found between diapausing and reproductive flies in monounsaturated alkenes, 4 and 45%, n-alkanes, 24 and 37%, monomethylalkanes, 57 and 15%, and dimethylalkanes, 15 and 2%, respectively, for females. A small difference was found in the total amount of hydrocarbon present, 7.3 ± 0.6 and 9.7 ± 1.1 μg/fly, between diapausing and reproductive female flies, respectively. Adult males also exhibited a similar change in hydrocarbon profile and amount between diapausing and reproductive flies. A laboratory strain of face flies originating in Minnesota was also analyzed, and again similar differences were observed in both male and female flies that were kept under a reproductive or diapause condition. Arch. Insect Biochem. Physiol. 37:206–214, 1998. © 1998 Wiley-Liss, Inc. |