Visualizing real time [11C]methionine translocation in Fe-sufficient and Fe-deficient barley using a Positron Emitting Tracer Imaging System (PETIS) |
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| Authors: | Nakanishi H; Bughio N; Matsuhashi S; Ishioka N; Uchida H; Tsuji A; Osa A; Sekine T; Kume T; Mori S |
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| Institution: | Laboratory of Plant Molecular Physiology, Department of Applied Biological Chemistry, The University of Tokyo, 1-1-1 Yoyoi, Bunkyo-ku, Tokyo 113-8657, Japan; Takasaki Radiation Chemistry Research Establishment, Japan Atomic Energy Research Institute, Gunma 370-1207, Japan; Department of Radioisotopes, Japan Atomic Energy Research Institute, Gunma 370-1207, Japan; Central Research Laboratory, Hamamatsu Photonics Co., Shizuoka 434-0041, Japan; Corresponding author; Fax: +81 3 5684 4822; E-mail: asmori@hongo.ecc.u-tokyo.ac.jp |
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| Abstract: | 11C]methionine was supplied to Fe-deficient and
Fe-sufficient barley plants through a single leaf, and real time
11C movement was monitored using a Positron Emitting
Tracer Imaging System (PETIS). In Fe-deficient plants,
11C]methionine was translocated from the tip of the
absorbing leaf to the 'discrimination centre' located at the base of the
shoot, and then retranslocated to all the chlorotic leaves within 60 min,
while a negligible amount was retranslocated to the roots. In Fe-sufficient
plants, methionine was translocated to the discrimination centre and then
only to the newest leaf on the main shoot within 60 min. A negligible
amount was also retranslocated to the roots. In conclusion, methionine from
the above-ground parts of a plant is not a precursor of mugineic acid under
Fe-deficiency. The discrimination centre is suggested to play a vital role
in the distribution of mineral elements and metabolites in graminaceous
monocots.Keywords:
11C]methionine, discrimination centre,
Fe deficiency, mugineic acid, PETIS.
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