Cloning and sequencing of the maltohexaose-producing amylase gene of Klebsiella pneumoniae |
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| Authors: | Momma M |
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| Institution: | Department of Biotechnology, National Institute of Agrobiological Resources, Ibaraki, Japan. momma@abr.affrc.go.jp |
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| Abstract: | The molecular characterization of the maltohexaose-producing amylase gene of Klebsiella pneumoniae revealed an open reading frame in which 2,031 base pairs encode a protein of 677 amino acids with a calculated molecular weight of 75,921. The amylase gene had high similarities of 73.6% in DNA sequence and 79.3% in deduced amino acid sequence with the periplasmic alpha-amylase MalS gene of Escherichia coli. |
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