Quantitative analysis of sulpiride in body fluids by high-performance liquid chromatography with fluorescence detection |
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Authors: | Gunnel Alfredsson Göran Sedvall Frits-Axel Wiesel |
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Institution: | Laboratory of Experimental Psychiatry, Department of Psychiatry, Karolinska Hospital, S-104 01 Stockholm Sweden |
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Abstract: | A high-performance liquid chromatographic method for the analysis of sulpiride, N-ethyl-2-(2-methoxy-5-sulphonamido-benzamido-methyl)-pyrrolidine, in body fluids is described. A structurally related compound, N-ethyl-2-(2,4-dimethoxy-benzamido-methyl)-pyrrolidine, was used as internal standard.A fluorescence detector with excitation maximum at 299 nm and emission maximum at 342 nm was used for the quantitation. The detection limit was about 10 ng/ml in serum and cerebrospinal fluid and about 200 ng/ml in urine. The experimental error was 5–10% in the concentration range 25–100 ng/ml. Some preliminary data from a pharmacokinetic study in healthy volunteers are presented. The half-life for sulpiride in serum was about 8 h. Sulpiride was also measured in cerebrospinal fluid from five drug-treated psychotic patients. |
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Keywords: | To whom correspondence should be addressed |
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