Effects of cadmium and zinc ions on purified lamb kidney cortex glucose-6-phosphate dehydrogenase activity |
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Authors: | Berivan Tandogan Nuray N Ulusu |
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Institution: | Hacettepe University, Faculty of Medicine, Department of Biochemistry, 06100, Ankara, Turkey |
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Abstract: | Glucose-6-phosphate dehydrogenase (G-6-PD) is the first enzyme in the pentose phosphate pathway. Cadmium is a toxic heavy metal that inhibits several enzymes. Zinc is an essential metal but overdoses of zinc have toxic effects on enzyme activities. In this study G-6-PD from lamb kidney cortex was competitively inhibited by zinc both with respect to glucose-6-phosphate (G-6-P) and NADP+ with Ki values of 1.066 ± 0.106 and 0.111 ± 0.007 mM respectively whereas cadmium was a non-competitive inhibitor with respect to both G-6-P and NADP+ Ki values of 2.028 ± 0.175 and 2.044 ± 0.289 mM respectively. |
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Keywords: | Glucose-6-phosphate dehydrogenase cadmium zinc inhibition |
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