甘蔗幼叶切段培养中的体细胞胚胎发生

本文从形态学和组织学方面研究了甘蔗幼叶胚性愈伤组织发生及体细胞胚胎的形成过程。甘蔗幼叶片切段培养于含2.4—D1.5mg/1的MS培养基上,4—6天后切段开始形成愈伤组织,约10天后愈伤组织表面出现白色颗粒状结构。将含有白色颗粒状结构的愈伤组织转移至不含激素的培养基中,7—10天后可见有小植株长出。组织学和形态学观察表明,甘蔗离体再生植株是通过体细胞胚胎发生途径。

SOMATIC EMBRYOGENESIS AND PLANT REGENERATION FROM LEAF TISSUES OF SUGARCANE

The formation of embryogenic callus and somatic embryogenesis from sugarcane ( Saccharum officinarum L. ) leaves were investigated. When cultured on medium supplemented with 2.4-dichlorphenoxyacetic acid ( 2.4-D ) 1.5/1, sucrose 3 %, segments of young leaves began to produce callus after 4-6 days in culture, and the amount of callus increased with the culture time. In about 10 days some white, compact nodular structures appeared on the surface of the callus. Histological studies showed that the white compact nodular structures were embryoids which appear-ed in the early developing stage. Transferred of the callus containing nodular structures to MS medium devoiding of plant hormones, perfect embryoids were formed which developed later into plantlets. It was suggested that the plants regenerated from the callus via somatic embryogenesis.