| 橡胶树体细胞胚发生过程中DNA甲基化分析 |
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| 引用本文： | 李辉亮,郭冬,彭世清.橡胶树体细胞胚发生过程中DNA甲基化分析[J].热带亚热带植物学报,2015,23(5):527-533. |
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| 作者姓名： | 李辉亮 郭冬 彭世清 |
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| 作者单位： | 中国热带农业科学院热带生物技术研究所, 农业部热带作物生物学与遗传资源利用重点实验室, 海口571101,中国热带农业科学院热带生物技术研究所, 农业部热带作物生物学与遗传资源利用重点实验室, 海口571101,中国热带农业科学院热带生物技术研究所, 农业部热带作物生物学与遗传资源利用重点实验室, 海口571101 |
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| 基金项目： | 国家自然科学基金项目(31170634); 海南省重大科技项目(ZDZX2013023-1)资助 |
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| 摘 要： | 为探讨巴西橡胶树(Hevea brasiliensis)自根幼态无性系与供体间差异产生的原因,应用甲基化敏感扩增多态性扩增技术,对巴西橡胶树体细胞胚发生过程中基因组DNA 胞嘧啶甲基化程度和模式进行了分析。结果表明,在巴西橡胶树体细胞胚发生过程中不同阶段的DNA 甲基化程度不同,以花药的DNA 甲基化程度最高,体细胞胚的DNA 甲基化水平最低。在体细胞胚发生过程中出现了I、Ⅱ和Ⅲ 3 种类型的甲基化多态性带型的改变,包括他们的出现与消失。因此,橡胶树体细胞胚发生过程中可能通过DNA 甲基化甲基化和去甲基化来调控基因的表达。
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| 关 键 词： | 橡胶树 体细胞胚 DNA 甲基化 甲基化敏感扩增多态性 |
| 收稿时间： | 2014/11/14 0:00:00 |
| 修稿时间： | 2015/3/12 0:00:00 |
DNA Methylation Changes during Somatic Embryogenesis of Hevea brasiliensis |
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| Li Hui-liang,Guo Dong and Peng Shi-Qing.DNA Methylation Changes during Somatic Embryogenesis of Hevea brasiliensis[J].Journal of Tropical and Subtropical Botany,2015,23(5):527-533. |
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| Authors: | Li Hui-liang Guo Dong and Peng Shi-Qing |
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| Institution: | Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China,Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China and Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China |
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| Abstract: | In order to understand the causes of differences between self-rooting juvenile clone and donors of Hevea brasiliensis, the extent and pattern of cytosine methylation in genomic DNA of H. brasiliensis were studied by using methylation-sensitive amplification polymorphism (MSAP). The results showed that MSAP profiles of genomic DNA were different during somatic embryogenesis. The genomic DNA methylation of anther was the highest among all tissues, while that of somatic embryo was the lowest. Changes in types of MSAP bands among different tissues were frequently observed, including appearance and disappearance of types I, II, and III MSAP bands. Therefore, the gene expression could be regulated by DNA methylation or demethylation during somatic embryogenesis of H. brasiliensis. |
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| Keywords: | Hevea brasiliensis Somatic embryogenesis DNA methylation Methylation-sensitive amplification polymorphism |
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