Regulation of glutamine synthetase in fed-batch cultures of Neurospora crassa. |
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Authors: | J Limón-Lason M Lara B Resendiz J Mora |
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Institution: | 1. Departmento de Biotecnologǐa Instituto de Investigaciones Biomédicas, U.N.A.M., México 20, D.F., Mexico;2. Departmento de y Biologǐa Molecular Instituto de Investigaciones Biomédicas, U.N.A.M., México 20, D.F., Mexico |
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Abstract: | The effect of the nitrogen and carbon sources in the regulation of gu tamine synthetase has been studied in fed-batch cultures of . The limitation of ammonium in an excess of the carbon source, leads to an accumulation of α-ketoglutarate and elevation of glutamine sy thetase. The limitation of sucrose in an excess of ammonium results in a decrease in glutamine synthetase activity. These results indicate that the carbon source exerts a positive control in the regulation of glutamine synthetase. |
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