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Regulation of glutamine synthetase in fed-batch cultures of Neurospora crassa.
Authors:J Limón-Lason  M Lara  B Resendiz  J Mora
Institution:1. Departmento de Biotecnologǐa Instituto de Investigaciones Biomédicas, U.N.A.M., México 20, D.F., Mexico;2. Departmento de y Biologǐa Molecular Instituto de Investigaciones Biomédicas, U.N.A.M., México 20, D.F., Mexico
Abstract:The effect of the nitrogen and carbon sources in the regulation of glu tamine synthetase has been studied in fed-batch cultures of Neurospora crassa. The limitation of ammonium in an excess of the carbon source, leads to an accumulation of α-ketoglutarate and elevation of glutamine syn thetase. The limitation of sucrose in an excess of ammonium results in a decrease in glutamine synthetase activity. These results indicate that the carbon source exerts a positive control in the regulation of glutamine synthetase.
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