| EB病毒转化Hu－TLC－SCID小鼠脾细胞的实验研究 |
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| 引用本文： | 左丽,郭辉玉,张昌卿.EB病毒转化Hu－TLC－SCID小鼠脾细胞的实验研究[J].Virologica Sinica,1996(2). |
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| 作者姓名： | 左丽 郭辉玉 张昌卿 |
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| 作者单位： | 中山医科大学微生物学教研室，中山医科大学肿瘤医院中心实验室，贵阳医学院微生物学教研室 |
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| 摘 要： | 利用ＥＢ病毒转化可产生较高水平人ＩｇＧ和特异性抗２型登革病毒人抗体的Ｈｕ－ＴＬＣ－ＳＣＩＤ小鼠脾细胞，通过免疫组化、免疫荧光和ＰＣＲ法检测转化细胞的人Ｂ细胞表面标志、ＥＢ病毒抗原和ＥＢ病毒基因。结果表明，被转化的Ｈｕ－ＴＬＣ－ＳＣＩＤ小鼠脾细胞能继续产生抗２型登革病毒的特异性人抗体，并具有人Ｂ细胞的、ＳｍＩｇＧ标志及ＥＢ病毒潜伏膜蛋白－１（ＬＭＰ－１）基因，可表达ＬＭＰ－１和ＥＢ病毒核抗原（ＥＢＮＡ）。
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| 关 键 词： | 人化SCID小鼠，EB病毒，转化作用 |
Transformation of Spleen Cells Prepared from Dengue Virus-2 Immunized Hu-TLC-SCID Mice |
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| Zuo Li,Guo Huiyu,et al..Transformation of Spleen Cells Prepared from Dengue Virus-2 Immunized Hu-TLC-SCID Mice[J].中国病毒学(英文版),1996(2). |
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| Authors: | Zuo Li Guo Huiyu |
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| Abstract: | he spleen cells isolated from SCID mice transplanted with human tonsillar lymphocytes(Hu- TLC-SCID)immunized with dengue virus 2 type(DV_2)in vitro and in vivo were trans-formed by Epstein-Barr virus(EBV).Specific human anti-DV_2 antibodies were detectable in thesupernatant of the transfomed spleen cells.The presence of human B lymphocyte markers(and SmIgG) as well as EBV antigens (LMP1 and BENA)and LMP1 gene fragment in the trans-formed spleen cells determined by immunoperoxidase staining immunofluorescence technique andPCR technique,indicated that the Hu-TLC-SCID mouse spleen cell has been successfully trans-foimed by EBV.The EBV-transformed Hu-TLC-SCID mouse spleen cell could be utilized forcontinous production of specific human anti-DV_2 antibodies in vitro. |
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| Keywords: | Hu-SCID mouse Epstein-Barr virus Transfoimation |
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