蓝舌病毒HbC3株对小鼠乳腺癌MA-782细胞的感染特性

体外研究蓝舌病毒湖北株3(BTV-HbC3)对鼠乳腺癌细胞MA782的感染性并探讨BTV-HbC3靶向性溶癌的机制。观察MA782细胞感染BTV-HbC3的病变效应(CPE);MTT法研究病毒致细胞病变率的特征;透射电镜观察感染病毒后细胞超微结构的变化;免疫组化研究病毒蛋白在细胞内的表达特点;凋亡染色(TUNEL法)观察病毒诱导细胞凋亡的情况;流式细胞仪测定病毒对MA782细胞周期的影响。结果证明BTV-HbC3感染MA782细胞后有明显的细胞病变效应;病毒蛋白主要表达在细胞的胞膜及胞浆内;凋亡染色发现大量的凋亡细胞;流式细胞仪可见明显的亚二倍体峰。故认为BTV-HbC3在体外能有效的感染MA782细胞,并能诱导MA782细胞凋亡。

The Infectivity of Bluetongue Virus Strain HbC3 to MA782 Cell

To investigate the characteritics and the mechanism of bluetongue virus strain HbC3 (BTV-HbC3) infecting MA782 malignancy in vitro,BTV-HbC3 was used to infect MA782 cell, and the cytopathic effect(sCPE)was observed.Transmission electron microscop(eTEM)was introduced to study changes of ultrastructure. Immunohistochemical staining was used to observe the expressed viral protein in tumors and organs. TUNEL staining was taken to detect the apoptosis of MA782 cells induced by BTV-HbC3. The results indicated that MA782 cell was sensitive; Lots of virtu particles were found in cytoplasm by TEM ;Viral protein expressed in cell membrane and cytoplasm; and early apoptopsis cells were found by TUNEL staining. All these resucss showed that BTV-HbC3 could infect and replicate in MA782 cells efficiently in vitro.