Gram-Positive Marine Bacteria as a Potential Resource for the Discovery of Quorum Sensing Inhibitors |
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Authors: | Margaret E Teasdale Kellye A Donovan Stephanie R Forschner-Dancause David C Rowley |
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Institution: | (1) Department of Biomedical and Pharmaceutical Sciences, University of Rhode Island, Kingston, RI 02881, USA;(2) Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, 53 Fogarty Hall, Kingston, RI 02881, USA; |
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Abstract: | Inhibitors of bacterial quorum sensing have been proposed as potentially novel therapeutics for the treatment of certain bacterial
diseases. We recently reported a marine Halobacillus salinus isolate that secretes secondary metabolites capable of quenching quorum sensing phenotypes in several Gram-negative reporter
strains. To investigate how widespread the production of such compounds may be in the marine bacterial environment, 332 Gram-positive
isolates from diverse habitats were tested for their ability to interfere with Vibrio harveyi bioluminescence, a cell signaling-regulated phenotype. Rapid assay methods were employed where environmental isolates were
propagated alongside the reporter strain. “Actives” were defined as bacteria that interfered with bioluminescence without
visible cell-killing effects (antibiotic activity). A total of 49 bacterial isolates interfered with bioluminescence production
in the assays. Metabolite extracts were generated from cultures of the active isolates, and 28 reproduced the bioluminescence
inhibition against V. harveyi. Of those 28, five extracts additionally inhibited violacein production by Chromobacterium violaceum. Chemical investigations revealed that phenethylamides and a cyclic dipeptide are two types of secondary metabolites responsible
for the observed activities. The active bacterial isolates belonged primarily to either the genus Bacillus or Halobacillus. The results suggest that Gram-positive marine bacteria are worthy of further investigation for the discovery of quorum sensing
antagonists. |
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