Anti-hepatitis B virus constituents from the stem bark of Streblus asper |
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| Institution: | 1. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Ministry of Education of China), School of Environment and Resource of Guangxi Normal University, Guilin 541004, PR China;2. Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), Guilin 541004, PR China;3. Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guilin 541004, PR China;4. Peoples’ Hospital of Pubei, Pubei 535300, PR China;1. Department of Neurobiology, Harvard College, Harvard University, Cambridge, MA, USA;2. Department of Biostatistics and Epidemiology, Medical College of Georgia, Augusta University, Augusta, GA, USA;3. Department of Medicine, Medical College of Georgia, Augusta University, Augusta, GA, USA;4. Cardio-Renal Physiology & Medicine, Division of Nephrology, Department of Medicine, University of Alabama at Birmingham, Birmingham, AL, USA;5. Georgia Prevention Institute, Department of Pediatrics, Medical College of Georgia, Augusta University, Augusta, GA, USA |
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| Abstract: | Seven compounds, (7′S,8′S)-trans-streblusol A, (7′R,8′S)-erythro-streblusol B, (7′S,8′S)-threo-streblusol B, 8′R-streblusol C, streblusquinone, (8R,8′R)-streblusol D, and streblusol E, along with 15 known compounds (8–22) were isolated from the n-butanol-soluble part of the MeOH extract of stem bark of Streblus asper. Their structures were elucidated through application of extensive spectroscopic methods, including ESI-MS and 2D NMR spectroscopy (HMQC and HMBC). The stereochemistry at the chiral centers was determined using CD spectra, as well as analyses of coupling constants and optical rotation data. The isolated lignans and allylbenzene derivatives were evaluated for their anti-HBV activities in vitro using the HBV transfected Hep G2.2.15 cell line. The most active compounds, magnolol and 9-β-xylopyranosyl-isolariciresinol, exhibited significant anti-HBV activities with IC50 values of 2.03 and 6.58 μM for secretion of HBsAg, with no cytotoxicity, and of 3.76 and 24.86 μM for secretion of HBeAg, with no cytotoxicity. |
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