Investigation of the Cytotoxicity of Antimicrobial Peptide P40 on Eukaryotic Cells |
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Authors: | Rodrigo A Vaucher Mario L Teixeira Adriano Brandelli |
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Institution: | 1. Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência de Alimentos, ICTA, Universidade Federal do Rio Grande do Sul, Av. Bento Gon?alves 9500, Porto Alegre, 91501-970, Brazil
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Abstract: | The in vitro cytotoxicity of the antimicrobial peptide P40 was investigated. The food grade bacteriocin nisin was also analyzed
for comparison. VERO cells were treated with different concentrations (0.02–2.5 μg ml−1) of nisin and P40, and cell viability and plasma membrane integrity were checked by MTT, neutral red uptake (NRU), and lactate
dehydrogenase (LDH) assays. In MTT and NRU assays the EC50 to the purified peptide P40 were 0.30 and 0.51 μg ml−1, while values found to nisin were 0.35 and 0.79 μg ml−1, respectively. In the LDH assay, the EC50 was 0.57 and 0.62 μg ml−1 for P40 and nisin, respectively. The peptide P40 revealed higher hemolytical activity (19%) when compared to nisin (4.9%)
at the highest concentration tested (2.5 μg ml−1). Relatively few studies about the cytotoxicity of antimicrobial peptides are available. The determination of the cytotoxicity
of antimicrobial peptides is an essential step to warrant their safe use. |
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