Isolation of the first trypsin inhibitor from the genusAspergillus |
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| Authors: | Rashda Ali Zafar H. Zaidi |
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| Affiliation: | (1) H. E. J. Research Institute of Chemistry, University of Karachi, 32 Karachi, Pakistan;(2) Present address: Department of Applied Chemistry, University of Karachi, 32 Karachi, Pakistan |
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| Abstract: | ![]()
The fungusAspergillus flavipes was grown on a Czapeck sucrose medium; the biomass so obtained was treated with high concentration of sucrose to release intracellular metabolites. Sephadex G-75 chromatography of the latter yielded a pure protein having anti-trypsin activity in vitro. |
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