Molecular cloning and expression patterns of FK506‐binding protein 12, an immunophilin from the cabbage butterfly,Pieris rapae |
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Authors: | Yong Hun Jo Dong Hyun Kim Seung Han Oh Bharat Bhusan Patnaik Ho Beom Lee Hun Cheon Jeong Yong Seok Lee Kisung Ko In Seok Bang Iksoo Kim Yeon Soo Han |
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Institution: | 1. Division of Plant Biotechnology, College of Agriculture and Life Science, Chonnam National University, 300 Yongbong‐dong, Buk‐gu, Gwangju 500‐757, Korea;2. Hampyeong County Insect Institute, Hampyeong County Agricultural Technology Center, Hampyeong, 525‐811, Korea;3. Department of Biological Science, Biotechnology Institute, College of Natural Sciences, Wonkwang University, Iksan, Korea;4. Department of Biological Science and the Research Institute for Basic Sciences, Hoseo University, Asan 336‐795, Republic of Korea |
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Abstract: | FK506‐binding protein (FK506BP) class belonging to immunophilin protein family has been known to play key roles in modulating T‐cell activation, regulation of cell cycle and protein folding. However, little is known about the involvement of FK506BP during viral pathogenesis in insect host. In this study, an attempt has been made to focus on the involvement of FK506BP in antiviral innate immunity, by cloning the full‐length cDNA of FK506BP12 (PrFK506BP12) from the cabbage butterfly, Pieris rapae. It comprised of 532 bp (excluding poly‐A tail) with a longest open reading frame (ORF) of 327 bp encoding 108 amino acids. In silico analysis of PrFK506BP12 ORF revealed a highly conserved FK506‐binding domain (FKBD). As expected, it showed high homology to other FK506BPs identified from Bombyx mori (92%), Manduca sexta (91%), Suberites domuncula (82%), Tribolium castaneum (81%) and Aedes aegypti (74%) . Expression of PrFK506BP12 was observed during developmental stages of P. rapae, but was pronounced in late pupal and adult stage. In addition, spatial expression pattern analysis indicated its high expression in the head and fat body. Furthermore, PrFK506BP12 mRNA was induced 12 h after LTA, Poly I:C treatment and 3h after Pieris rapae granulovirus (PrGV) treatment in carcass. It suggests that PrFK506BP12 appears to be involved in immune responses and also play an important role in the fat body, although it remains to be clarified about their precise role in response to granulovirus. |
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Keywords: | Pieris rapae granulovirus immunophilin FK506BP12 |
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