Utilization of sodium-dependent high affinity choline uptake in vitro as a measure of the activity of cholinergic neurons in vivo. |
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Authors: | S Atweh J R Simon M J Kuhar |
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Institution: | 1. Departments of Pharmacology and Experimental Therapeutics USA;2. Psychiatry and the Behavioral Sciences The Johns Hopkins University School of Medicine Baltimore, Maryland 21205, USA |
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Abstract: | Previous reports indicate that alterations of activity of cholinergic neurons are followed by parallel changes in sodium-dependent high affinity choline uptake . These results are consistent with the proposal that this portion of choline uptake is regulatory in the synthesis of ACh. These results also suggest the possibility of utilizing sodium-dependent high affinity choline uptake as a measure of the relative state of cholinergic activity . In this study, we administer a number of drugs reported to alter turnover and release of ACh (both are measures of cholinergic activity , and subsequently examine sodium-dependent high affinity choline uptake . Administration of pentobarbital, chloral hydrate, morphine, physostigmine, Δ9 THC, hemicholinium-3 and oxotremorine, drugs which decrease ACh turnover and release, caused a reduction in choline uptake. Conversely, administration of pentylenetetrazol, atropine, scopolamine, and haloperidol, drugs which increase ACh turnover and release, caused an increase in choline uptake . These findings support the proposal that sodium-dependent high affinity choline uptake can be used as a relative measure of the activity of cholinergic neurons . |
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